血管平滑肌細(xì)胞是許多動脈疾病的主要靶細(xì)胞。血管平滑肌細(xì)胞的加速生長潛能是血管疾病進(jìn)展的關(guān)鍵因素。*新研究表明血管平滑肌細(xì)胞表達(dá)的血管內(nèi)皮細(xì)胞表達(dá)細(xì)胞粘附因子ICAM-1 和 VCAM-1，研究表明這些因子能促進(jìn)血管內(nèi)皮細(xì)胞增殖，分化和血管壁的炎癥反應(yīng)，并且與血管疾病的進(jìn)展及穩(wěn)定有關(guān)。人血管平滑肌細(xì)胞的體外培養(yǎng)是血管研究中的重要模型，并將對血管疾病的藥理學(xué)和治療研究提供大量信息。
ScienCell實驗室的HBVSMC分離自人大腦組織，原代凍存，冰凍運輸。每管細(xì)胞密度超過5×105/ml。細(xì)胞經(jīng)α-smooth muscle actin和desmin免疫熒光鑒定。本細(xì)胞經(jīng)檢測不含HIV-1、HBV、HCV、支原體、細(xì)菌、酵母菌和真菌。如采用ScienCell 實驗室特制的培養(yǎng)基，可保證此細(xì)胞達(dá)到15次或以上的倍增。
推薦培養(yǎng)基：平滑肌細(xì)胞培養(yǎng)基 (SMCM, Cat. #1101)
1.) Kikkawa Y, Matsuo S, Kurogi R, Nakamizo A, Mizoguchi M, Sasaki T. (2014) 'Upregulation of Relaxin after Experimental Subarachnoid Hemorrhage in Rabbits.'
2.) Shi X, Fu Y, Liao D, Chen Y, Liu J. (2014) 'Alterations of Voltage-Dependent Calcium Channel Currents in Basilar Artery Smooth Muscle Cells at Early Stage of Subarachnoid Hemorrhage in a Rabbit Model.'
3.) Kikkawa Y, Matsuo S, Kurogi R, Nakamizo A, Mizoguchi M, Sasaki T. (2014) "Upregulation of Relaxin after Experimental Subarachnoid Hemorrhage in Rabbits." Biomed res int. 2014: 836397.
4.) Shi X, Fu Y, Liao D, Chen Y, Liu J. (2014) "Alterations of Voltage-Dependent Calcium Channel Currents in Basilar Artery Smooth Muscle Cells at Early Stage of Subarachnoid Hemorrhage in a Rabbit Model." PloS one. 9: e84129.
5.) Cucullo L, Hossain M, Tierney M, Janigro D. (2013) "A new dynamic in vitro modular capillaries-venules modular system: cerebrovascular physiology in a box." BMC Neurosci. 14: 18.
6.) Meng H, Zhang X, Lee SJ, Wang MM. (2013) "Von Willebrand Factor Inhibits Mature Smooth Muscle Gene Expression through Impairment of Notch Signaling." PLoS One. 8: e75808.
7.) Miners JS, Kehoe P, Love S. (2011). "Neprilysin protects against cerebral amyloid angiopathy and Abeta-induced degeneration of cerebrovascular smooth muscle cells."Brain Patholology. 21: 594-605
8.) Fossati S, Cam J, Meyerson J, Mezhericher E, Romero IA, Couraud PO, Weksler BB, Ghiso J, Rostagno A. (2010) "Differential activation of mitochondrial apoptotic pathways by vasculotropic amyloid-beta variants in cells composing the cerebral vessel walls." FASEB J. 24: 229-41.
9.) Lefterov I, Fitz NF, Cronican AA, Fogg A, Lefterov P, Kodali R, Wetzel R, Koldamova R. (2010) "Apolipoprotein A-I deficiency increases cerebral amyloid angiopathy and cognitive deficits in APP/PS1DeltaE9 mice." J Biol Chem 285: 36945-57.
10.) Kanekiyo T, Bu G. (2009) "Receptor-associated protein interacts with amyloid-beta peptide and promotes its cellular uptake." J Biol Chem. 284: 33352-59.
11.) Wang J, Tuo H, Wang R, Li W, Feng Z, Chen J, Wang D. (2008) "The expression of human brain vascular smooth muscle cell AT receptor after the UL83 gene of HCMV inhibition by small interfering RNAs." Neurol Res. 30: 903-09.
