貨號(hào)	1700
產(chǎn)地	美國
縮寫	HSC
規(guī)格	5 x 10^5 /1ml
用途	科研
儲(chǔ)存	液氮
運(yùn)輸	干冰
關(guān)鍵字	人雪旺細(xì)胞 HSC  人雪旺細(xì)胞 HSC  人雪旺細(xì)胞 HSC  人雪旺細(xì)胞 HSC  人雪旺細(xì)胞 HSC  人雪旺細(xì)胞 HSC  人雪旺細(xì)胞 HSC
公司簡(jiǎn)介	合肥星肽生物科技有限公司作為專業(yè)的ScienCell中國代理商，專業(yè)經(jīng)營(yíng)實(shí)驗(yàn)室科研用原代細(xì)胞、原代細(xì)胞專用培養(yǎng)基、原代細(xì)胞無血清培養(yǎng)基、干細(xì)胞、干細(xì)胞培養(yǎng)基、干細(xì)胞無血清培養(yǎng)基的研究和開發(fā)。合肥星肽生物科技有限公司始終致力于為客戶提供可靠的研究材料以及方便快捷的服務(wù)，對(duì)購買項(xiàng)目的前期資料提供,中期合同保證,后期貨物跟蹤到*終售后的確保項(xiàng)目準(zhǔn)確到位，都有相關(guān)專業(yè)人士進(jìn)行維護(hù),確保您在合肥合肥星肽生物科技有限公司公司獲得*上等服務(wù)！
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1.) Cho YR, Lim JH, Kim MY, Kim TW, Hong BY, Kim YS, Chang YS, Kim HW, Park CW. (2014) 'Therapeutic Effects of Fenofibrate on Diabetic Peripheral Neuropathy by Improving Endothelial and Neural Survival in db/db Mice.'

2.) Cho YR, Lim JH, Kim MY, Kim TW, Hong BY, Kim YS, Chang YS, Kim HW, Park CW. (2014) "Therapeutic Effects of Fenofibrate on Diabetic Peripheral Neuropathy by Improving Endothelial and Neural Survival in db/db Mice." PloS one. 9: e83204.

3.) Guerreiro LTA, Robottom-Ferreira AB, Ribeiro-Alves M, Toledo-Pinto TG, Brito TR, Rosa PS, Sandoval FG, Jardim MR, Antunes SG, Shannon EJ, Sarno EN, Pessolani MCV, Williams DL, Moraes MO. (2013) "Gene expression profiling specifies chemokine, mitochondrial and lipid metabolism signatures in leprosy." PLoS One. 8: e64748.

4.) Das A, Shergill U, Thakur L, Sinha S, Urrutia R, Mukhopadhyay D, Shah VH. (2013) "Ephrin B2/EphB4 pathway in hepatic stellate cells stimulates Erk-dependent VEGF production and sinusoidal endothelial cell recruitment." Am J Physiol Gastrointest Liver Physiol. 298: G908-15.

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6.) Stavniichuk R, Obrosov AA, Drel VR, Nadler JL, Obrosova IG, Yorek MA. (2013) '12/15-Lipoxygenase inhibition counteracts MAPK phosphorylation in mouse and cell culture models of diabetic peripheral neuropathy.'

7.) Stavniichuk R, Obrosov AA, Drel VR, Nadler JL, Obrosova IG, Yorek MA. (2013) "12/15-Lipoxygenase inhibition counteracts MAPK phosphorylation in mouse and cell culture models of diabetic peripheral neuropathy." J diabetes mellitus. 3.

8.) Peng J, Wang Y, Zhang L, Zhao B, Zhao Z, Chen J, Guo Q, Liu S, Sui X, Xu W, Lu S. (2011) "Human umbilical cord Wharton's jelly-derived mesenchymal stem cells differentiate into a Schwann-cell phenotype and promote neurite outgrowth in vitro." Brain Res Bull. 84: 235-43.

9.) Ahmad Z, Brown CM, Patel AK, Ryan AF, Ongkeko R, Doherty JK. (2010) "Merlin knockdown in human Schwann cells: clues to vestibular schwannoma tumorigenesis." Otol Neurotol. 31: 460-66.

10.) Arima Y, Hayashi H, Kamata K, Goto TM, Sasaki M, Kuramochi A, Saya H. (2010) "Decreased expression of neurofibromin contributes to epithelial-mesenchymal transition in neurofibromatosis type 1." Exp Dermatol. 19: e136-41.

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17.) Bodempudi V, Yamoutpoor F, Pan W, Dudek AZ, Esfandyari T, Piedra M, Babovick-Vuksanovic D, Woo RA, Mautner VF, Kluwe L, Clapp DW, De Vries GH, Thomas SL, Kurtz A, Parada LF, Farassati F. (2009) "Ral overactivation in malignant peripheral nerve sheath tumors." Mol Cell Biol. 29: 3964-74.

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1、Question:

What is the best way you suggest to cryopreserve primary human Schwann cells?

Answers:

We do not recommend that customers re-freeze our cells since primary cells are fragile and they may be damaged during re-freeze and re-thaw process.
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2、Question:

In the product description, these cells were analyzed by immunofluorescence using antibodies against S100, GFAP, and CD90. Would you be able to provide manufacturer/catalog# for those antibodies? Your protocols for cell preparation and immunofluorescence using those antibodies would be kindly appreciated as well? Thank you

Answers:

Posted by Manoj Sharma on Thursday, January 14, 2016 Dear Amish, Here are the antibodies we use for staining #1700 Human Schwann Cells: （1）S-100 beta: Sigma Cat. No. S2532 1:500 dilution （2）GFAP: Sigma Cat. No. G3893 1:1000 dilution And following is the protocol that we use for staining the cells. Immunofluorescence Staining Protocol a. Fixing the slide i. Wash each well with 500 μl PBS making sure to add along the side of the well wall. ii. Add 500 μl 4% PFA (paraformaldehyde) to each well and place at room temperature for 5 minutes. b. Blocking Solution i. Wash each well with 500 μl PBS. ii. The blocking solution consists of 5% NGS (Normal Goat Serum) and 0.1% Triton-X-100 in PBS. iii. The blocking solution can be made in bulk in a 15mL conical tube and then placed in the wells. iv. For every 1 ml of PBS add 50 μl of NGS and 10 μl of 10% Triton-X 100. Add 250 μl per well and incubate at room temperature for 1 hour. c. Primary Antibody i. Prepare the solution for the primary antibody. This consists of 1% NGS and 1% Triton-X 100 in PBS. For every 1 ml of PBS add 10 μl of NGS and 10 μl of Triton-X 100. This can be made in bulk in a conical tube. ii. Dilute each primary antibody needed with the necessary dilution factor (depending on the antibody used) and add 250 μl to the appropriate wells. Place the slide in 4°C overnight. Dilutions can be made in 1.5mL microcentrifuge tubes. iii. If the slide needs be stained on the same day, it can be placed in the 37°C incubator for 2 hours instead of 4°C overnight. iv. Make sure to note on the Immunocytochemistry form the specific antibody placed in the well. d. Secondary Antibody i. Wash the slide 3 times with 500 μl PBS leaving the PBS in the wells 5-7 minutes per rinse. ii. Dilute each secondary antibody (usually 1:1000 to 1:2000, depending on the intensity) needed with PBS and add 250 μl to the appropriate wells. Incubate at 37°C in the incubator for 45 minutes. iii. Wash the slide 5 times with 500 μl PBS leaving the PBS in the wells 5-7 minutes per rinse. iv. The secondary antibody is specific to the primary antibody such as IgG Rabbit or IgG Mouse. The animal it derives from must be the same as the primary antibody. e. Preparing the slide for microscopic examination i. Drop about 5-8 μl of mounting medium (DAPI) to a slide, remove cover slips from the well using tweezers and place them over the slide on the mounting medium gently. ii. Make sure the DAPI covers the entire area of the cover slips. f. Microscopic Examination Examine the slides after 20-30 minutes under fluorescence microscope. Thank you for your interest in ScienCell Products
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3、Question:

Has anyone used these cells to look at myelin production? I work with a virus that may disrupt myelination and I was interested in looking at this in primary tissue culture cells. Thanks! Lee

Answers:

We do not test for myelin production. thank you for your interest.
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