視網膜是位于眼睛后部的多層結構。它包括感光細胞和視網膜色素上皮細胞（RPEpiC）。RPEpiC位于神經視網膜和脈絡膜之間，形成視網膜外屏障，控制著視網膜下腔的化學構成。人們發現RPEpiC內有許多特定偏振分布的細胞內蛋白質。與其他上皮細胞通常頂生面沒有基質相比，RPEpiC的頂生面胞膜是與細胞外基質相接觸的。RPEpiC在調節相鄰的感光膜周轉、維生素A的代謝、保護細胞內黑色素所致的視網膜光損害中起關鍵作用。RPEpiC與很多視網膜疾病的發病相關，如增殖性視網膜病變和年齡相關性黃斑變性。
Sciencell研究實驗室的HRPEpiC是從人視網膜分離得到的，原代凍存，冰凍運輸。每管細胞密度超過5×10^5/ml。細胞特性經cytokeratine -18, -19和纖維粘連蛋白免疫熒光鑒定。本細胞經檢測不含HIV-1、HBV、HCV、支原體、細菌、酵母菌和真菌。如采用ScienCell 實驗室特制的培養基，可保證此細胞10倍增增殖。
推薦培養基：上皮細胞培養基 (EpiCM, Cat. #4101)
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貨號
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6540
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產地
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美國
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縮寫
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HRPEpiC
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規格
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5 x 10^5 /1ml
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用途
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科研
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儲存
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液氮
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運輸
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干冰
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關鍵字
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人視網膜色素上皮細胞 HRPEpiC 人視網膜色素上皮細胞 HRPEpiC 人視網膜色素上皮細胞 HRPEpiC 人視網膜色素上皮細胞 HRPEpiC 人視網膜色素上皮細胞 HRPEpiC 人視網膜色素上皮細胞 HRPEpiC 人視網膜色素上皮細胞 HRPEpiC 人視網膜色素上皮細胞 HRPEpiC
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公司簡介
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合肥星肽生物科技有限公司作為專業的ScienCell中國代理商，專業經營實驗室科研用原代細胞、原代細胞專用培養基、原代細胞無血清培養基、干細胞、干細胞培養基、干細胞無血清培養基的研究和開發。合肥星肽生物科技有限公司始終致力于為客戶提供可靠的研究材料以及方便快捷的服務，對購買項目的前期資料提供,中期合同保證,后期貨物跟蹤到 *終售后的確保項目準確到位，都有相關專業人士進行維護,確保您在合肥合肥星肽生物科技有限公司公司獲得*上等服務！
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聯系方式
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何經理，合肥星肽生物科技有限公司，Tel：0551-63802898 400-8702-898，
E-mail：info@qingbio.com http://www.28888123.cn QQ:514713116
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1.) Favret S, Binet F, Lapalme E, Leboeuf D, Carbadillo J, Rubic T, Picard E, Mawambo G, Tetreault N, Jean- Joyal S, Chemtob S, Sennlaub F, SanGiovanni JP, Guimond M,Sapieha P. (2013) "Deficiency in the metabolite receptor SUCNR1 (GPR91) leads to outer retinal lesions." Aging (Albany NY). 5: 427.
2.) Miyamoto N, Izumi H, Miyamoto R, Bin H, Kondo H, Tawara A, Sasaguri Y, Kohno K. (2011) "Transcriptional Regulation of Activating Transcription Factor 4 under Oxidative Stress in Retinal Pigment Epithelial ARPE-19/HPV-16 Cells." Invest Ophthalmol Vis Sci. 52: 1226-34.
3.) Narayan S, Prasanna G, Tchedre K, Krishnamoorthy R, Yorio T. (2010) "Thrombin-induced endothelin-1 synthesis and secretion in retinal pigment epithelial cells is rho kinase dependent." J Ocul Pharmacol Ther. 26: 389-97.
4.) Bertram KM, Baglole CJ, Phipps RP, Libby RT. (2009) "Molecular regulation of cigarette smoke induced-oxidative stress in human retinal pigment epithelial cells: implications for age-related macular degeneration." Am J Physiol Cell Physiol. 297: C1200-10.
5.) Mannermaa E, Vellonen KS, Ryh?nen T, Kokkonen K, Ranta VP, Kaarniranta K, Urtti A. (2009) "Efflux protein expression in human retinal pigment epithelium cell lines."Pharm Res. 26: 1785-91.
6.) Cheong HI, Johnson J, Cormier M, Hosseini K. (2008) "In vitro cytotoxicity of eight beta-blockers in human corneal epithelial and retinal pigment epithelial cell lines: comparison with epidermal keratinocytes and dermal fibroblasts." Toxicol In Vitro. 22: 1070-6.
7.) Swaney JS, Moreno KM, Gentile AM, Sabbadini RA, Stoller GL. (2008) "Sphingosine-1-phosphate (S1P) is a novel fibrotic mediator in the eye." Exp Eye Res. 87: 367-75.
8.) Hunt DW, Boivin WA, Fairley LA, Jovanovic MM, King DE, Salmon RA, Utting OB. (2006) "Ultraviolet B light stimulates interleukin-20 expression by human epithelial keratinocytes." Photochem Photobiol. 82: 1292-300.
Question:
Regarding Human Retinal Pigment Epithelial Cells Catalog #6540 Are the cells from a single human donor or multiple in a pool? If single donor, can one order vials from a few donors to compare? Can you provide a list of available donors and their characteristics? I have read the following paper where this product is used and they make the comment that they conducted their work with hRPEpC in multiple donors. Toxicol Sci. 2015 Mar;144(1):163-72. Retinal Toxicity, in vivo and in vitro, Associated with Inhibition of Nicotinamide Phosphoribosyltransferase.
Answers:
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Hi Elizabeth,
it is Single donor.if you are interested in multiple vials from different lots to get different donors. Please contact us at techsupport@sciencellonline.com to obtain donor information.
Thanks for your interest in our products.
SCIENCELL TEAM
